| 摘要 |
The field of the invention is recombinant production of carboxypeptidase B. This invention provides a modified prodomain of carboxypeptidase B which enhances recombinant expression thereof when co-expressed from a separate gene. Preferred modified prodomains have added amino acids at their C-terminus, in particular any one of the following sequences: L, KDEL, KKAA or SDYQRL. The carboxypeptidase is preferably human pancreatic carboxypeptidase B. The invention also relates to corresponding polynucleotide sequences, vectors, host cells and methods of recombinant carboxypeptidase B production.
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