Method of isolation and purification of fusion polypeptides
摘要
Methods for isolation and purification of recombinant proteins are described. Fusion proteins incorporating a cleavage site sensitive to proteolysis by a plant virus proteinase may be cleaved from carrier proteins to provide high yields of protein product. Methods employing a plant virus proteinase to cleave expressed fusion proteins are particularly suitable for obtaining proteolytically sensitive polypeptides in the presence of added cell protease inhibitors. Also disclosed are recombinant vectors useful for overproducing plant virus proteinases in a suitable host.
申请公布号
US5532142(A)
申请公布日期
1996.07.02
申请号
US19930021603
申请日期
1993.02.12
申请人
BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM;STATE OF OREGON, THE, ACTING BY AND THROUGH THE OREGON STATE SYSTEM OF HIGHER EDUCATION, ON BEHALF OF OREGON STATE UNIVERSITY