Process of producing beta -lactam antibiotics applying microorganisms with increased ligase activity

摘要

The present invention relates to the biosynthesis of beta-lactam antibiotics. More specifically, the invention relates to processes of producing beta-lactam antibiotics in vivo and in vitro. Also contemplated is a novel enzyme capable of catalyzing certain steps involved in beta-lactam biosynthesis. Further, the invention relates to a DNA construct encoding the novel enzyme, a recombinant vector or transformation vehicle comprising the DNA construct, and finally a cell comprising the DNA construct or recombinant vector.