Alkyne-activated fluorogenic azide compounds and methods of use thereof

摘要

The present disclosure provides fluorogenic azide compounds. Also provided are methods of using the subject compounds for labelling a target biomolecule that includes an alkyne. In some embodiments, the method includes contacting the biomolecule with a fluorogenic azide compound, wherein the contacting results in covalent linkage of the compound with the alkyne moiety of the target biomolecule.

主权项

1. A compound of any one of formulae (II), (IV) and (V): wherein R1, R2, R3, R4 and R5 are each independently selected from the group consisting of hydrogen, alkyl, substituted alkyl, alkoxy, substituted alkoxy, azido, amino, substituted amino, nitro, cyano, acyl, carboxyl, carboxylester, cycloalkyl, substituted cycloalkyl, heterocycloalkyl, substituted heterocycloalkyl, aryl, substituted aryl, heteroaryl, and substituted heteroaryl; optionally, one of R1, R2, R3, R4 or R5 together with its adjacent R-group form a cycloalkyl, substituted cycloalkyl, heterocycloalkyl, substituted heterocycloalkyl, aryl, substituted aryl, heteroaryl or substituted heteroaryl; and one of R1, R2, R3, R4 or R5 is azido and one of R1, R2, R3, R4 or R5 is optionally a molecule of interest.