Specific and high affinity binding proteins comprising modified SH3 domains of Fyn kinase

摘要

The present invention relates to a method for the production of a library comprising recombinant derivatives of the SH3 domain of the Fyn kinase of SEQ ID NO: 1 as well as a method for selecting from a library comprising recombinant derivatives of the SH3 domain of the Fyn kinase of SEQ ID NO: 1 one or more of said derivatives having a specific binding affinity to a protein or peptide.

主权项

1. A method for the production of a library comprising recombinant derivatives of the SH3 domain of the Fyn kinase of SEQ ID NO: 1, said derivatives having a specific binding affinity to a protein or peptide, wherein said protein or peptide is not a natural SH3 binding ligand, wherein said method comprises the steps of
(a) generating recombinant derivatives of the SH3 domain of the Fyn kinase of SEQ ID NO: 1 by substituting at least one amino acid in the RT loop of SEQ ID NO: 1, and substituting at least one amino acid in the src loop of SEQ ID NO: 1, and (b) constructing a library comprising the recombinant derivatives of the SH3 domain generated in step (a); wherein the recombinant derivatives of the SH3 domain of the Fyn kinase of SEQ ID NO: 1 retain at least 80% sequence identity to the amino acid sequence of SEQ ID NO: 1, and retain at least 90% identity to the amino acid sequence of SEQ ID NO: 1 outside the src and RT loops; and wherein the src loop is located at amino acid positions 31 to 34 of SEQ ID NO: 1, and the RT loop is located at amino acid positions 12 to 17 of SEQ ID NO: 1.