| 摘要 |
PROBLEM TO BE SOLVED: To inhibit the reduction of amplification efficiency even if a cycle time is shortened.SOLUTION: In the present invention, a denatured step of transferring and staying a droplet 20 to a first region 36A of a container 10 heated to the denaturation temperature of a target nucleic acid, and a cycle passing through a synthetic step of transferring and staying the droplet 20 to a second region 36B of a container 10 different from the first region 36A are repeated multiple times. A fluorescent-labeling probe is contained in the droplet 20, and the fluorescent-labeling probe contains a minor groove binder molecule.SELECTED DRAWING: Figure 7 |
