Mechanisms of Antimicrobial Susceptibility

摘要

Disclosed herein are methods and compositions for determining the presence or absence of a mechanism of antimicrobial resistance in a sample.

主权项

1. A method for determining the presence or absence of a mechanism of antimicrobial resistance in a microorganism that is not susceptible to an antimicrobial in a sample, comprising:
providing a sample comprising a microorganism of interest; contacting a portion of said sample with an inhibitor, wherein said inhibitor inhibits a mechanism of conferring non-susceptibility in said microorganism; contacting said portion of said sample comprising said inhibitor with an antimicrobial agent, wherein said antimicrobial agent is a compound that kills, inhibits the growth, or otherwise compromises the viability of one or more microorganisms; contacting said portion of said sample with a live cell reporter capable of generating a detectable signal; and detecting the presence or absence of a signal from said portion of said sample, wherein the presence of said signal indicates that the mechanism of antimicrobial resistance inhibited by said inhibitor is not present in said microorganism, and wherein the absence of said signal indicates that the mechanism of antimicrobial resistance inhibited by said inhibitor is present in said microorganism.