método para determinar a presença de um marcador biológico luminescente de produção de fóton, kit para realizar o método e uso de pontos quânticos não ligados a um marcador biológico

摘要

The present invention relates a method to determine the presence of a photon producing biological marker in a cell, tissue or organism of interest. The method is based on Fluorescence by Unbound Excitation from Luminescence (FUEL) and comprises the steps of
a) providing conditions suitable for the biological marker to produce at least one first photon by luminescence
b) providing a FUEL probe pair-upper (FPP-U) disposed in proximity to the cell, tissue or organism, wherein the at least one first photon of step a) excites the FPP-U, which emits at least one second photon.
The FPP-U may be selected from the group of quantum dots, carbon nanotubes, fluorescent proteins, diamond nanocrystals, and metalloporphyrins. This method is characterized in that said biological marker and said FPP-U are not bound and in that each of the at least one second photon (s) are of a longer wavelength than each of the at least one first photon (s).