Method and kit for the classification and prognosis of chronic wounds

摘要

A method and kit are provided for identifying non-healing or healing chronic mammalian wound tissue or for determining the prognosis of chronic mammalian wound tissue based upon the identification of at least one key set of molecular markers or genes whose expression pattern is indicative of a given wound type and so representative of a given prognosis.

主权项

1. A method for identifying abnormal or non-healing chronic mammalian would tissue, which method comprises:
a) amplifying, by PCR, a combination of molecular markers Psoriasin, Claudin-5, IL8RB, IL22R, PTPRK, TEM4, TEM7R, VEGF-C, ARP2, and CARI from said sample of wound tissue; b) determining the level of expression of said combination of said molecular markers; and c) concluding, when an increased level of expression of each of the molecular markers compared to a control is seen, that the wound is an abnormal or non-healing chronic wound; wherein said step of amplification step (a) is performed with the primer sets:Psoriasin:SEQ ID NO: 37, SEQ ID NO: 38;Claudin-5:SEQ ID 21, SEQ ID NO: 22;IL8RB:SEQ ID NO: 19, SEQ ID NO: 20;IL22R:SEQ ID NO: 15, SEQ ID NO: 16;PTPRK:SEQ ID NO: 25, SEQ ID NO: 26;TEM4:SEQ ID NO: 41, SEQ ID NO: 42;TEM7R:SEQ ID NO: 23, SEQ ID NO: 24;VEGF-C:SEQ ID NO: 7, SEQ ID NO: 8;ARP2:SEQ ID NO: 1, SEQ ID NO: 2;CAR1:SEQ ID NO: 47, SEQ ID NO: 48;Endomucin:SEQ ID NO: 13, SEQ ID NO: 14;IL7BR:SEQ ID NO: 43, SEQ ID NO: 44;KaI1:SEQ ID NO: 45, SEQ ID NO: 46; andCREBL1:SEQ ID NO: 49, SEQ ID NO: 50.