摘要 |
FIELD: agriculture.SUBSTANCE: invention relates to agricultural biotechnology. The invention is a method of reproduction of tea tick trefoil, when the matured sterilized seeds of tea tick trefoil (Hedysarum theinum Krasnob) are planted in the Murashige-Skoog culture medium for insertion into tissue of the culture, in 20-30 days the developed shoots are transplanted to the MS medium of reproduction, then the formed conglomerates of microshoots are divided and transplanted to the media comprising 1.0 BAP + L-glutamine and adenine sulphate 100 mg/l. The plants-regenerants are reproduced, rooted and adapted to non-sterile conditions.EFFECT: invention enables to improve the efficiency of the process, replicability, adaptation under conditions of hydroponics, and to obtain standard seedlings of tea tick trefoil.4 dwg, 1 tbl |