| 摘要 |
<P>PROBLEM TO BE SOLVED: To provide a composition for freeze preservation, which freezes and preserves a human mesenchymal stem cell and a human fat-derived stem cell applicable for regenerative medicine without using a harmful freeze preservative such as dimethyl sulfoxide, etc., or an animal-derived component. <P>SOLUTION: An ε-poly-L-lysine is reacted with succinic anhydride to replace ≥60% of amino group of the ε-poly-L-lysine with a carboxy group. The polymer compound thus obtained is dissolved in a Dulbecco's modified eagle's medium (DMEM) being one of culture solution on the market in 10 w/w% to give a freeze preservation solution having low toxicity. A polyhydric alcohol such as glycerin, ethylene glycol, propylene glycol, etc., and a polymer compound such as dextran, polyethylene glycol, etc., are added to the freeze preservation solution to further improve a preservation ratio. <P>COPYRIGHT: (C)2011,JPO&INPIT |
