Synthesis of fluorescent noble metal nanoparticles

摘要

A process for the production of fluorescent nanoparticles selected from noble metal or silica nanoparticles. Noble metals or silica nanoparticles provide a platform to which fluorophores and other detection molecules can be added. The fluorescent nanoparticles can be used as regents, diagnostic assays and in the tracking or targeting of tumors.

主权项

1. A process for the production of noble metal or silica nanoparticles as probes capable to fluoresce to label cells and tissues, the method comprising:(1) providing a platform of the nanoparticles;(2) covering the surfaces of the nanoparticles to saturation with thiol-terminated polymers by one of the following methods:
(a) mixing the nanoparticles with methoxy-(polyethylene glycol)-thiol, the methoxy-(polyethylene glycol)-thiol having a MW of about 10,000 atomic mass units (amu), and biotin-(polyethylene glycol)-thiol, the biotin-(polyethylene glycol)-thiol having a MW of about 10,000 amu; (b) mixing the nanoparticles with fluorescently-labeled methoxy-(polyethylene glycol)-thiol, the methoxy-(polyethylene glycol)-thiol having a MW of about 10,000 amu and biotin-(polyethylene glycol)-thiol, the biotin-(polyethylene glycol)-thiol having a MW of about 10,000 amu; (c) coordinating thiol and biotin thiol to the surfaces of the nanoparticles by a non-covalent bond; and (d) directly conjugating methoxy-thiol and biotin-thiol to the surfaces of the nanoparticles,whereby the polymers bind to the surfaces of the nanoparticles as a brush layer via thiol particle coordination of the thiol ends so that the biotin or methoxy ends are free, thereby obtaining polymer-covered nanoparticles;(3) homogeneously mixing the resulting polymer-covered nanoparticles with fluorophore labeled avidin or an avidin derivative thereof in proportions of 1 biotin molecule for every 10 to 1000 avidin molecules thereby obtaining fluorescent multi-coloured nanoparticle-avidin complexes and the fluorophore labeled avidin or a derivative thereof being spaced away from the surface of the noble metal or silica nanoparticles, thus reducing or removing the potential quenching of the fluorophore.