DRUG PROMOTING CERAMIDE TRANSPORT, BASE SEQUENCE FOR PRODUCING THE DRUG, METHOD OF MEASURING ACTIVITY OF PROMOTING CERAMIDE RELEASE AND METHOD OF MEASURING ACTIVITY OF PROMOTING INTERMEMBRANE CERAMIDE TRANSFER

摘要

A drug promoting ceramide transport which contains a protein having the amino acid sequence of SEQ ID NO:1 as the active ingredient; the base sequence of SEQ ID NO:3; a method of measuring an activity of promoting ceramide release which comprises mixing a lipid membrane containing ceramide with a drug promoting ceramide release, centrifuging the thus obtained mixture to give a supernatant and measuring the ceramide content in the supernatant; and a method of measuring an activity of promoting intermembrane ceramide transfer which comprises mixing an acceptor membrane, a drug promoting ceramide transport and a donor membrane containing ceramide, adding a selective membrane aggregation agent thereto, then separating the acceptor membrane from the donor membrane, and measuring ceramide contents in the acceptor membrane and in the donor membrane. Thus, it is possible to provide a drug promoting ceramide transport, a base sequence for producing the drug, a method of measuring an activity of promoting ceramide release and a method of measuring an activity of promoting transmembrane ceramide transfer.