METHOD AND DEVICE FOR INTEGRATED NUCLEIC ACID INTEGRITY ASSESSMENT AND ANALYSIS

摘要

The present invention relates to methods for integrated ribonucleic acid integrity assessment and analysis comprising the steps of: (a) providing a support having immobilized thereon a set of detector probes and a set of at least two control probes, wherein (i) a first of said at least two control probes is complementary to 23S or 28S rRNA or a functionally equivalent RNA, and (ii) a second of said at least two control probes is complementary to 16S or 18S10 rRNA or a functionally equivalent RNA; (b) contacting said support with analyte ribonucleic acids derived from a sample, under conditions allowing hybridisation of complementary analyte ribonucleic acids and immobilized probes to form analyte ribonucleic acid/probe complexes; wherein said hybridisation generates a detectable signal;15 (c) detecting signals generated by said complexes; (d) determining the ratio of signals generated by the 28S/control probe and 18S rRNA/control probe or 23S rRNA/control probe and 16S rRNA/control probe or functionally equivalent rRNA/control probe complexes; (e) assessing the integrity of said sample; and20 (f) evaluating microarray analysis results in view of said integrity assessment. The present invention further relates to use of said methods as well as microarrays, devices comprising said microarrays, kits for carrying out said methods and computer products.