Assay for testing a sample for the presence or absence of inhibition of the enzymatic conversion of 1-hydroxy-2-methyl-(E)-butenyl 4-diphosphate into isopentenyl diphosphate and/or dimethylallyl diphosphate by the following steps: a) reacting an aqueous mixture containing 1-hydroxy-2-methyl-(E)-butenyl 4-diphosphate, a 1-hydroxy-2-methyl-(E)-butenyl 4-diphosphate reductase, and a reducing agent under predetermined reaction conditions for a predetermined period of time; b) analyzing the reaction mixture obtained in step (a) for the consumed amount of 1-hydroxy-2-methyl-(E)-butenyl 4-diphosphate and/or NAD(P)H and/or for the produced amount of isopentenyl diphosphate, and/or dimethylallyl diphosphate and/or NAD(P)<+>; c) repeating step (a) in the presence of the sample to be tested; d) repeating step (b) with the reaction mixture defined in step (c); e) comparing the results of steps (b) and (d).
申请公布号
WO2004065623(A2)
申请公布日期
2004.08.05
申请号
WO2004EP00264
申请日期
2004.01.15
申请人
BACHER, ADELBERT;ADAM, PETRA;EISENREICH, WOLFGANG;GRAEWERT, TOBIAS;HECHT, STEFAN;ROHDICH, FELIX;ZEPECK, FERDINAND
发明人
BACHER, ADELBERT;ADAM, PETRA;EISENREICH, WOLFGANG;GRAEWERT, TOBIAS;HECHT, STEFAN;ROHDICH, FELIX;ZEPECK, FERDINAND