发明名称 |
Process for lysing mycobacteria |
摘要 |
A process for lysing Mycobacteria to liberate nucleic acids such as DNA and RNA comprises heating the Mycobacteria in an aqueous solution to lyse the Mycobacteria and release the nucleic acids, with the aqueous solution containing a chelating agent such as EDTA or EGTA in an amount effective to inhibit degradation of the released nucleic acids. Examples of Mycobacteria which can be lysed include Mycobacterium fortuitum and Mycobacterium tuberculosis. After lysis, the nucleic acid is preferably then amplified and detected. Kits useful for carrying out the present invention are also disclosed.
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申请公布号 |
US5856145(A) |
申请公布日期 |
1999.01.05 |
申请号 |
US19970901375 |
申请日期 |
1997.07.28 |
申请人 |
BECTON, DICKINSON AND COMPANY |
发明人 |
DOWN, JAMES A.;FRAISER, MELINDA S.;WALKER, G. TERRANCE |
分类号 |
C12N15/09;C12N1/06;C12N1/20;C12N15/00;C12P19/34;C12Q1/04;C12Q1/68;C12R1/33;(IPC1-7):C12P19/34 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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