| 摘要 |
A non-ionic lytic agent, preferably saponin for reducing the background carbon dioxide produced by blood cell metabolism is used in the testing of cultures for the presence of microorganisms. The hemolytic agent saponin is combined with a phospholipid, preferably L- alpha -Lecithin (phosphatidylcholine), to form mixed micelles which protect saponin from the effects of heat sterilization and high blood cholesterol levels, thus maintaining the lytic activity of saponin. The phospholipid/saponin mixed micelles are added to non-radiometric culture media vials such as Bactec TM NR6A, NR7A and NR8A. The media vials are used in the Bactec TM NR-660 and NR-730 instruments. However, the present invention may also be used in radiometric media such as Bactec TM culture vials 6, 7 and 8 for reducing background carbon dioxide levels detected in C14 radiometric instruments such as the Bactec TM -460, or in other instruments designed to detect bacteria in the presence of mammalian cells by monitoring the increase in CO2 produced by the metabolizing bacteria.
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