| 摘要 |
PURPOSE:To obtain L-lysine in high yield, by cultivating a variant, belonging to the genus Brevibacterium or Corynebacterium, having lower activities of citric acid synthesizing enzyme than a parent strain and no demanding properties for L- glutamic acid. CONSTITUTION:A variant having L-lysine producing cmpetence, derived from Brevibacterium devaricatum ATCC14020 as a parent strain, etc., belonging to the genus of Brevibacterium or Corynebacterium, having lower activities of citric acid synthesizing enzyme than the parent strain, possessing no L-glutamic acid demanding properties, is cultivated. The cultivation is carried out using an ordinary nutrient medium conventionally. It is done under aerobic conditions at 24-37 deg.C in pH5-9 for 2-7 days, and L-lysine is collected from the culture solution by a method, e.g., ordinary ion exchange resin method, etc. |
