发明名称 |
Human multipotent embryonic stem cell-like progenitor cells |
摘要 |
The invention provides a plurality of embryonic stem cell-like progenitor cells, which are isolated from a human tissue by a systemic screening of human mesenchymal stromal stem/progenitor cells and a cell sorting by a cell antigen selected from the group consisting of CD34, CD117, CD133, CD201, GloboH and combination thereof, and cultured in a medium supplemented with at least one or more steroids and one or more growth factors. The cells of the invention express CD34 and exhibit sphere-like clonogenicity in early passages and express multipotent embryonic stem cells (ESCs) like characteristics. |
申请公布号 |
US9234177(B2) |
申请公布日期 |
2016.01.12 |
申请号 |
US201113276106 |
申请日期 |
2011.10.18 |
申请人 |
SUNSHINE LIFE SCIENCE & TECHNOLOGY CORP. |
发明人 |
Tzu-Bi Shih Daniel;Tsai Ming-Song |
分类号 |
C12N5/0775;C12N5/073 |
主分类号 |
C12N5/0775 |
代理机构 |
Ditthavong & Steiner, P.C. |
代理人 |
Ditthavong & Steiner, P.C. |
主权项 |
1. A method for producing an enriched population of multipotent human CD34+ mesenchymal common progenitor cells (CD34+ MCPCs), further having at least the following characteristics: CD14+, CD117+, CD133+ (AC133+), CD201+, Nestin+, SSEA3+, SSEA4+ and GloboH+, the method comprising:
isolating from a human somatic tissue by a systemic screening of human mesenchymal stromal stem/progenitor cells followed by a cell sorting by a cell antigen of CD34, wherein the human somatic tissue is selected from the group consisting of amnion, endometrium and gingival; and culturing in a medium comprising EGF and hydrocortisone or a medium comprising bFGF and EGF. |
地址 |
New Taipei TW |