发明名称 |
Cell culture model for acquired chemoresistance of chronic myelogenous leukemia and related methods for identifying agents to overcome resistance |
摘要 |
A method of generating a chronic myelogenic leukemia (CML) acquired chemoresistant culture model is provided. Such a method may comprise providing a naïve blast crisis CML cell line; administering/contacting the cell line with a mutation-inducing dose of imatinib; maintaining a culture of the treated cell line for a period of time until the treated cell line relapses and repopulates the culture; and determining the repopulated cell culture is a CML acquired chemoresistant cell line by detecting a BCR-ABL mutation, wherein the acquired chemoresistance is achieved by a BCR-ABL mutation. |
申请公布号 |
US9176114(B2) |
申请公布日期 |
2015.11.03 |
申请号 |
US201313922141 |
申请日期 |
2013.06.19 |
申请人 |
CITY OF HOPE |
发明人 |
Chen WenYong;Bhatia Ravi;Su Leila;Yuan Yate-Ching |
分类号 |
A61K35/14;C12N5/0786;C12N5/09;C12P19/34;G01N33/50 |
主分类号 |
A61K35/14 |
代理机构 |
Perkins Coie LLP |
代理人 |
Perkins Coie LLP ;Dueppen Laura J. |
主权项 |
1. A culture model of chronic myelogenic leukemia (CML) acquired chemoresistant cells, the model generated by:
contacting a blast crisis CML cell line, which has previously not been exposed to imatinab before, with a dose of imatinib sufficient to induce a BCR-ABL mutation, wherein the blast crisis CML cell line is a KCL-22 cell line; maintaining a culture of the imatinib-treated cell line for about two to three weeks until the cell line relapses and repopulates the culture; and determining that the repopulated cell culture is a CML acquired chemoresistant cell line by detecting a BCR-ABL mutation. |
地址 |
Duarte CA US |