BIOMARKER COMPOSITIONS AND METHODS

摘要

Biomarkers can be assessed for diagnostic, therapy-related or prognostic methods to identify phenotypes, such as a condition or disease, or the stage or progression of a disease, select candidate treatment regimens for diseases, conditions, disease stages, and stages of a condition, and to determine treatment efficacy. Circulating biomarkers from a bodily fluid can be used in profiling of physiological states or determining phenotypes. These include nucleic acids, protein, and circulating structures such as vesicles, and nucleic acid-protein complexes. The invention provides methods of assessing microvesicles in a biological sample. The invention also provides an aptamer to a microvesicle surface antigen. The aptamer may be used for therapeutic purposes.

主权项

1. A method of detecting a presence or level of one or more microvesicle in a biological sample, comprising:
(a) contacting a biological sample with a lipid staining dye, wherein the biological sample comprises or is suspected to comprise the one or more microvesicle; and (b) detecting the lipid staining dye in contact with the one or more microvesicle, thereby detecting the presence or level of the one or more microvesicle.