| 发明名称 |
METHOD OF PREPARATION OF CULTURES OF SULPHIDE-BEARING BACTERIA FOR DNA EXTRACTION |
| 摘要 |
FIELD: biotechnologies.SUBSTANCE: method of preparation of cultures of sulphide-bearing bacteria for DNA extraction is offered. In the method 15 ml of cultural liquid are used. The cultural liquid is centrifuged at 1000 rpm. The triple washing of cells with phosphatic and salt buffer in the ratio 10:1 is performed. Mixing is performed within 10 seconds and centrifugation is conducted at 1000 rpm within 10 minutes.EFFECT: high efficiency of DNA extraction and possibility of fast and exact conducting of subsequent molecular and biological analysis.2 tbl, 2 ex |
| 申请公布号 |
RU2562176(C1) |
申请公布日期 |
2015.09.10 |
| 申请号 |
RU20140146906 |
申请日期 |
2014.11.22 |
| 申请人 |
FEDERALNOE GOSUDARSTVENNOE AVTONOMNOE OBRAZOVATELNOE UCHREZHDENIE VYSSHEGO OBRAZOVANIYA NATSIONALNYJ ISSLEDOVATELSKIJ TOMSKIJ GOSUDARSTVENNYJ UNIVERSITET |
发明人 |
KARNACHUK OLGA VIKTOROVNA;FRANK YULIYA ALEKSANDROVNA;GERASIMCHUK ANNA LEONIDOVNA;LUSHCHAEVA INNA VLADIMIROVNA |
| 分类号 |
C12N15/10;C12N1/20;C12P19/34 |
主分类号 |
C12N15/10 |
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