Temperature-controlled electrospray ionization source and methods of use thereof

摘要

Disclosed herein is an electrospray ionization source that provides improved temperature control compared to prior sources. A combination of a continuous flow sample design and the use of a long heat shield combine to improve thermal control and reduce memory effects observed with prior designs. The temperature-controlled source is particularly useful for the study of biomolecules, particularly the study of protein aggregation.

主权项

1. A temperature-controlled electrospray ionization source, comprising
a metallic capillary for transport of a sample, the metallic capillary connected to a sample injector at a first end and connected to a spray emitter at a second end, wherein the metallic capillary has a capillary length for the transport of the sample from the first end to the second end of the metallic capillary, and wherein the inner diameter of the spray emitter is substantially the same at both ends of the spray emitter, a metallic heat shield in thermal contact with the spray emitter, wherein the metallic heat shield surrounds the spray emitter and extends along the spray emitter length from the first end to the second end, of the spray emitter and a heating element in thermal contact with the metallic capillary and the metallic heat shield, wherein the metallic capillary is configured to connect to a voltage source, and the sample injector is configured to be in communication with a pump to infuse the sample into the metallic capillary at a flow rate of 0.01 to 100 μL/min.