Methods and means for enhancing skin transplantation using gene delivery vehicles having tropism for primary fibroblasts, as well as other uses thereof

摘要

The present invention relates to providing human primary fibroblasts with a nucleic acid of interest with, among others, the purpose to improve the taking of, for example, skin transplants, particularly, methods of transducing fibroblasts with the nucleic acid of interest by means of gene delivery vehicles, in particular chimeric recombinant adenovirus-based (having an improved tropism for human primary fibroblasts) gene delivery vehicles. The present invention is exemplified by an adenovirus serotype 5 genome-based vector with an adenoviral fiber protein of a B-type or a D-type adenovirus, in particular adenovirus type 40 or 16, and wherein the nucleic acid of interest encodes a protein which improves angiogenesis and/or neurovascularization, in particular myogenin or MyoD.