The present invention provides plasmids for insertional mutagenesis in bacteria. Specifically, the invention provides plasmids comprising a transposition system including a transposon and a transposase, in which transposon insertion can be temporally regulated. The plasmids can be used to determine the relative importance of a particular gene in viability of the organism from which the gene is derived. The plasmids are particularly useful for large-scale screening of a multiplicity of genes.
申请公布号
WO02068631(A2)
申请公布日期
2002.09.06
申请号
WO2002US05776
申请日期
2002.02.28
申请人
GENOME THERAPEUTICS CORPORATION;SHAW, KAREN, J.;KENNEY, TERESA;SHIMER, GEORGE, H., JR.
发明人
SHAW, KAREN, J.;KENNEY, TERESA;SHIMER, GEORGE, H., JR.