| 摘要 |
The invention concerns a method for separating and superconcentrating of small molecules in liquid phase, by passing them through a biologically active porous membrane (5). This method consists in the following steps: producing enzymatic pumping of the product to be concentrated (Pac) through the membrane (5), delimiting two basins one for starting liquid CI and for final liquid for CII. A couple of reversing enzymes E1/E2 are provided, E1 being fixed on the face of the membrane (5) opposite CI and E2 on the other face; causing E1 or E2 to transform the Pac into an intermediate migrant (IM) capable of passing through the membrane (5) to be subsequently transformed into Pac by the reversing enzyme CII. Liquids CI/CII are moved to produce non-turbulent diffusion layers adjacent to the two faces of the membrane (5); in the event the IM is electrically charged, a current of a kind opposite to that of this IM is provided for the membrane, whereas in the event the IM is neutral, the membrane (5) is provided with the same electric charge as that of the Pac. Finally the accumulated and superconcentrated Pac is collected in CII. The invention also concerns the separator/concentrator reactor (1) for implementing said method. |
