| 摘要 |
<p>Polyamines of formula (1a) and (1b) and their salts are new. R = H, R2 = NH-(CH2)4-NH-(CH2)3-NH2 or R1 = 0H, R2 = -NH-(CH2)4-NH-(CH2)3-NH2 or R1 = 0H, R2 = -CH2-NH-(CH2)3-NH2 R3 = R4 = 0H, R5 = H or R5 = 0H, R3 = R4 = H. Also new are polypeptides characterised by (a)- present in a fraction from crude venom of the Agelenopsis aperta spider which elutes off a C-18 Vydac (RTM) 22 mm x 250 mm, 300 A pore size, 10 micron particle size column using a flow rate of 15 ml/min and a solvent system using a linear gradient program of 5% -20% B, 95% - 80% A (0-30 min) then 20%-70%B, 80%-30 A (30-55 min), where A is 0.1 aw TFA and B is acetonitrile, at about 22.75 minutes: (b) present in a fraction of the fraction described in (a), which elutes off a C-18 Vydac (RTM) 22 mm x 250 mm, 300 A pore size, 10 micron particle size column using a flow rate of 15ml/min and a solvent system using a non-linear gradient program of 0% -10% B, 100% - 100% A (0-5 min), then 0% - 10% B, 100% - 90% A (5-20 min) (waters curve 1) then 10% - 20% B, 90-80% A (20-30 min). (Waters curve 6) then 20% - 50% B, 80%-50% A (30-40 min). (Waters curve 11), where A is 0.1% aq TFA and B is acetonitrile, at about 21.5 mins; and (c) FAB mS: high resolution: 505.3861.</p> |
