| 摘要 |
PROBLEM TO BE SOLVED: To measure an antigen with accuracy without requiring the labeling of antibodies nor the separation of unreacted antibodies from reacted antibodies by measuring the variation of the surface charges of the antibodies which occur when an antigen-antibody complex is formed by causing a reaction between antibodies and antigens. SOLUTION: By causing a mixture produced as a result of an antigen-antibody reaction to make electrophoresis 5 by applying a voltage across a cell 1 containing the mixture and, at the same time, irradiating the cell 1 with light from a light source 2, such as an He-Ne laser, etc., perpendicularly to the direction of the electrophoresis, the then scattered light and reference light from the light source 2 led by means of mirrors 4a-4d are mixed and the frequency shifting amount of the scattered light from an antigen-antibody complex or an antibody is measured by detecting the shifting amount by means of a photoreceptor 3. Since the shifting amount is proportional to the moving speed of the complex, the mobility of the complex obtained by dividing the shifting amount by an electric field varies depending upon the state of surface charges. Therefore, an antigen can be determined by measuring the area or height of the peak of the spectrum related to the mobility of the antigen-antibody complex thus obtained and utilizing a calibration curve drawn by utilizing known amounts of antibodies. |
