DIFFERENTIAL TIMING METHOD FOR DETECTING MULTIPLE ANALYTES IN A TEST SAMPLE

摘要

The instant invention provides immunoassay methods whereby the presence or amount of multiple analytes that may be present in a test sample can be detected. According to one embodiment, the method comprises the steps of: (a) contacting a test sample with a common capture reagent for a time and under conditions sufficient to form capture reagent/analyte complexes wherein the common capture reagent includes one or more specific binding members that immobilize at least two analytes that may be present in said test sample; (b) contacting the capture reagent/analyte complexes with at least two indicator reagents for a time and under conditions sufficient to form capture reagent/analyte/indicator reagent complexes; and (c) detecting at least two measurable signals as a measure of the presence or amount of the analytes in the test sample. Indicator reagents which are employed in the above embodiment can comprise detectable moieties from at least two distinct detectable moiety classes. The invention also applies to detecting multiple nucleic acid sequences which may be present in a test sample.