PROTEIN, CORRESPONDING TO A MATURE REGION OF MORPHOGENETIC PROTEIN IN HUMAN MP-52, PHARMACEUTICAL COMPOSITION FOR TREATING OF CARTILAGE AND BONE DISEASES AND METHOD FOR PREPARING THEREOF

摘要

1. A protein, corresponding to a mature region of morphogenetic protein in human MP-52 having the following structure: Pro-Leu-Ala-Thr SEQ ID NO 1). 2. A homodimer protein according to Claim 1. 3. A pharmaceutical composition for treating cartilage and bone diseases comprising a therapeutically effective amount of the protein according to Claim 2 and a pharmaceutically acceptable carrier. 4. The pharmaceutical composition according to Claim 3 wherein cartilage and bone diseases are osteoarthritis or arthrosteitis. 5. The pharmaceutical composition according to Claim 3 wherein cartilage and bone diseases are osteoporosis. 6. The pharmaceutical composition according to Claim 3 wherein cartilage and bone diseases are bone fracture and bone defect. 7. The pharmaceutical composition according to Claim 3 wherein cartilage and bone diseases are radicular and arvecular defects. 8. A process for preparing the protein of Claim 1 which comprises culturing E. coli transformed with a plasmid containing DNA sequence ATG CCA CTA GCA ACT CGT CAG GGC AAG CGA CCC AGC AAG AAC CTT AAG GCT CGC TGC AGT CGG AAG GCA CTG CAT GTC AAG TTC AAG GAC ATG GGC TGG GAC GAC TGG ATC ATC GCA CCC CTT GAG TAC GAG GCT TTC CAC TGC GAG GGG CTG TGC GAG TTC CCA TTG CGC TCC CAC CTG GAG CCC ACG AAT CAT GCA GTC ATC CAG ACC CTG ATG AAC TCC ATG GAC CCC GAG TCC ACA CCA Pro Thr Cys Cys Val Pro Thr Arg Leu Ser Pro Ile Ser Ile Leu Phe ATT GAC TCT GCC AAC AAC GTG GTG TAT AAG CAG TAT GAG GAC ATG GTC GTG GAG TCG TGT GGC TGC AGG, coding said protein with a methionine at the N-terminus, introducing the plasmid into E. Coli for transformation, solubilizing inclusion bodies obtained by culturing said E. Coli and purifying the monomer protein from the solubilized solution. 9. A process for preparing the dimer protein according to Claim 2 which comprises the steps of: constructing a plasmid containing DNA structure of which is defined in Claim 8; transforming E. coli by this plasmid culturing said E. coli, solubilizing inclusion bodies obtained by culturing said E. coli, purifying the monomer protein from the solubilized solution, refolding the monomer protein into a dimer protein and purifying the same. 10. A method for treating cartilage and bone diseases, which comprises administering to a human a pharmaceutical composition as claimed in Claim 3. 11. The method for treating cartilage and bone disease as claimed in Claim 11, wherein said disease is osteoarthritis or arthrosteitis. 12. The method for treating cartilage and bone diseases as claimed in Claim 11, wherein said disease is osteoporosis. 13. The method for treating cartilage and bone diseases as claimed in Claim 11, wherein said disease is bone fracture and bone defect. 14. The method for treating cartilage and bone diseases as claimed in Claim 11, wherein said disease is radicular and arvecular defects.