Chromatography matrices including novel <i>Staphylococcus aureus </i>protein a based ligands

摘要

The present invention relates to chromatography matrices including ligands based on one or more domains of immunoglobulin-binding proteins such as, Staphylococcus aureus Protein A (SpA), as well as methods of using the same.

主权项

1. An affinity chromatography ligand comprising two or more B domains or two or more C domains or two or more Z domains of Staphylococcus aureus Protein A (SPA), wherein each domain has a deletion of 3 or 4 consecutive amino acids from the N-terminus, starting at position 1 or position 2 corresponding to its wild type counterpart, wherein the ligand is immobilized onto a solid support and wherein the ligand exhibits reduced fragmentation following exposure of the ligand to 0.5 M NaOH for at least 5 hours, as evidenced by SDS-PAGE and/or SEC techniques, relative to its wildtype counterpart.