| 摘要 |
A method for the identification of a polynucleotide encoding an enzyme of interest in a microfluidic device by providing an emulsion of microfluidic droplets comprising a library of polynucleotides encoding one or more enzyme, introducing a concentrated PCR solution which allows PCR amplification of the polynucleotides into selected droplets and/or introducing a lethal solution into deselected droplets, and identifying a polynucleotide encoding an enzyme of interest. |
