HIGH PH PROTEIN REFOLDING METHODS

摘要

Provided herein are methods for refolding denatured protein (e.g., from inclusion bodies) that do not require the use of a denaturing agent. Exemplary methods use a high pH for solubilizing denatured protein, followed by a decrease in pH for refolding the proteins.

主权项

1. A method for refolding a denatured protein, comprising
suspending a denatured protein in a suspension solution to obtain a composition comprising suspended denatured proteins; combining the composition comprising suspended denatured proteins with a solubilization buffer having a pH in the range of 10.5 to 13 to thereby obtain a composition comprising solubilized denatured proteins; and combining the composition comprising solubilized denatured proteins with a refold buffer having a pH in the range of 9 to 11 to thereby obtain a composition comprising refolded proteins; wherein the method does not include the use of a significant amount of denaturing agent.