| 摘要 |
A proteinaceous substance is rendered less soluble or less dispersible in an aqueous liquid by adding to said liquid with the proteinaceous substance dissolved or colloidally dispersed therein, polyethylene glycol having a molecular weight of at least 300 to precipitate at least some of said proteinaceous substance. The proteins may be mixed and fractional precipitation of the proteins is brought about by the addition of the polyethylene glycol. Aqueous polyethylene glycol of suitable concentration may be used for selective elution of precipitated protein fractions. The average molecular weight of the polyethylene glycol may be up to 100,000. The temperature may be from 0 DEG to 40 DEG C. and the process may be used in conjunction with pH control and inorganic salt precipitation. Examples relate to the fractionation of human blood plasma and the purification of Clostridium Novyi toxin, Botulinus Toxin type D, Tetanus anti-toxin, pancreatic desoxyribonuclease, fibrinogen and gamma globulin. In general, the process is applicable to the separation, isolation and purification of plasma proteins, anti-toxins, bacterial toxins, viruses, nucleic acid and enzymes.ALSO:A proteinaceous substance is rendered less soluble or less dispersable in an aqueous liquid by adding to said liquid with the proteinaceous substance dissolved or colloidally dispersed therein, polyethylene glycol having a molecular weight of at least 300 to precipitate at least some of said proteinaceous substance. The proteins may be mixed and fractional precipitation of the proteins is brought about by the addition of the polyethylene glycol. Aqueous polyethylene glycol of suitable concentration may be used for selective elution of precipitated protein fractions. The average molecular weight of the polyethylene glycol may be up to 100,000. The temperature may be from 0 DEG C to 40 DEG C. and the process may be used in conjunction with pH control and inorganic salt precipitation. Examples relate to the fractionation of human blood plasma and the purification of Clostridium Novyi toxin, Botulinus Toxin type D, Tetanus anti-toxin, pancreatic desoxyribonuclease, fibrinogen and gamma-globulin. In general, the process is applicable to the separation, isolation and purification of plasma proteins, antitoxins, bacterial toxins, viruses, nucleic acid and enzymes.
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