| 摘要 |
<p>Universal solid support oligonucleotide synthesis reagents, oligonucleotide synthesis processes, and reagents for cleaving oligonucleotides from solid supports are disclosed. Oligonucleotide synthesis reagents have the following general formula: SS - R6 - O - R3 wherein SS is a solid support; R6 is (a), (b) or (c), where R5 is hydrogen or alkyl and R4 is a phosphate protecting group; and R3 is a ring moiety having vicinal groups -XR1 and -YR2 wherein each of X and Y is independently selected from the group consisting of O, S and NH and one of R?1 and R2¿ is a blocking moiety and the other is hydrogen or a hydroxy protecting group. Oligonucleotide cleaving reagents include methylamine and/or ammonium hydroxide and trimethylamine.</p> |
