| 摘要 |
<p>The invention relates to medicine. The inventive biomaterial producing method consists in sawing a natural cleaned bone into plates whose thickness ranges from 0.2 to 2.0 cm, in washing said plates by 0.1 phosphate-pH 5.8-6.0 buffer heated to 65 °C, in overcooking in an of activated 0.1-0.4 % papain solution at a temperature of 65 °C for 24 hours, in subsequently washing the plates by 5 water volumes at a temperature ranging from 40 to 80 °C, in treating by 0.4 n-alkali liquor at a room temperature during 10-24 hours, in washing by running water, in degreasing in the ethanol/chloroform mixtures, first, with a ratio of 1:2 and afterwards of 2:1, in decalcifying in 0.4 N-hydrochloric acid, in treating by 1.5-3 % hydrogen peroxide during 4 hours, in washing by purified water and, afterwards, by ethanol, in drying at a room temperature, in packing and sterilising. The thus obtainable material for osteoplasty and tissue engineering is embodied in the form a compound which contains a preserved native spatial organisation of a collagen matrix and the mineral constituent of a natural bone tissue comprising 25 % collagen, 75 % mineral substance and, according to a dry material analysis, non-collagen proteins in a quantity equal to or less that 1 %.</p> |
