| 发明名称 | Modulation of replicative fitness by deoptimization of synonymous codons | ||
| 摘要 | Methods of producing a pathogen with reduced replicative fitness are disclosed, as are attenuated pathogens produced using the methods. In particular examples, the method includes deoptimizing one or more codons in a coding sequence, thereby reducing the replicative fitness of the pathogen. Methods of using the attenuated pathogens as immunogenic compositions are also disclosed. | ||
| 申请公布号 | US8846051(B2) | 申请公布日期 | 2014.09.30 |
| 申请号 | US200511576941 | 申请日期 | 2005.10.07 |
| 申请人 | The United States of America as represented by the Secretary of the Department of Health and Human Services, Centers for Disease Control and Prevention | 发明人 | Kew Olen M.;Burns Cara C.;Shaw Jing;Campagnoli Raymond;Quay Jacqueline |
| 分类号 | A61K39/12;A61K39/21;A61K39/145;A61K39/155;A61K39/193;A61K39/215;A61K39/00;C12N7/00;C12N15/67;C07K14/245 | 主分类号 | A61K39/12 |
| 代理机构 | Klarquist Sparkman, LLP | 代理人 | Klarquist Sparkman, LLP |
| 主权项 | 1. A method of reducing replicative fitness of a virus, comprising: preparing a viral nucleic acid molecule comprising at least twenty deoptimized codons in a coding sequence of the virus by replacing each of the at least twenty codons in the coding sequence with a synonymous codon less frequently used in the virus, thereby generating a deoptimized viral nucleic acid molecule that reduces replicative fitness of the virus. | ||
| 地址 | Washington DC US | ||