CATIONIC WHEY PROTEIN COMPOSITION

摘要

Cationic proteins from whey were isolated using industrial scale chromatography and tested for cancer cell growth inhibition using [3H] -thymidine uptake as an indicator of DNA synthesis and cell growth. A dose-response relationship was evident in the range tested (215 µg/ml to 21,500 µg/ml). Colon cancer cells (CaCo-2), prostate cancer cells (LnCap), lung cancer cells (A549) and skin melanoma cells (B16-F1O) showed the most susceptibility to cationic protein inhibition. Mammary cancer cells (MDA-MB345) and brain cancer cells (U-87) also showed inhibition but a higher concentration of protein was needed to obtain equivalent inhibition. Cationic proteins are potent inhibitors of cancer cell growth.