Expression systems utilising the CH2-CH3 region of human immunoglobulin to target production of recombinant antibodies in avian eggs

摘要

An expression system for producing an antibody comprises a DNA sequence encoding a human immunoglobin heavy chain region and a regulatory region sufficient to provide expression of the antibody. The immunoglobin heavy chain region can bind to an avian egg and be internalized into yolk. The expression system may further comprise a DNA sequence encoding a human immunoglobin variable region. The antibody can be produced by introducing the expression system into an egg-laying animal, obtaining an egg containing the antibody and then the antibody can be isolated from the egg. Also disclosed is a transformed avian cell line that secretes the recombinant antibody and a transgenic egg-laying animal whose cells contain the expression system.